JAIC 1992, Volume 31, Number 3, Article 4 (pp. 313 to 323)
JAIC online
Journal of the American Institute for Conservation
JAIC 1992, Volume 31, Number 3, Article 4 (pp. 313 to 323)

AN INVESTIGATION INTO THE REMOVAL OF ENZYMES FROM PAPER FOLLOWING CONSERVATION TREATMENT

THERESA MEYER ANDREWS, WILLIAM W. ANDREWS, & CATHLEEN BAKER


ABSTRACT—The efficiency of rinsing two different α-amylases out of paper after conservation treatment was measured using radioactively labeled enzymes. The radioactive enzymes were used to treat small samples of Whatman and Japanese papers. The papers were subjected to various rinsing procedures, and the radioactivity remaining in the paper samples was determined. Buffers were used to maintain the pH of the enzyme solutions, and their effect in the removability of the enzymes was analyzed. Two water rinses were shown to be sufficient in removing more than 99% of both enzymes tested under optimal conditions. It was also found that increasing the concentration of the enzyme solution resulted in an increase in retention of enzymes in the paper.

Article Sections:

1. INTRODUCTION
2. EXPERIMENTAL MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSIONS
a: Notes , Materials , References , Author Information
Entire Article

Copyright 1992 American Institute for Conservation of Historic and Artistic Works