GAS CHROMATOGRAPHIC ANALYSIS OF AMINO ACIDS AS ETHYL CHLOROFORMATE DERIVATIVES.
MICHAEL R. SCHILLING, & HERANT P. KHANJIAN
ABSTRACT—Proteinaceous binding media present in paintings can be identified by gas chromato-graphic analysis of the ethyl chloroformate derivatives of amino acids. The effects of pigments on the amino acid composition data of hydrolyzed gelatin that were obtained by this method were studied. The method was also used to analyze glue and egg tempera paint films that were exposed to heat and light in order to accelerate the aging of the paints. Pigments and accelerated aging were found to affect the concentrations of all amino acids, although the concentrations of alkyl and imino-substituted amino acids were affected to a much smaller degree than others. Samples of grounds and paints that were removed from a number of paintings and painted objects were analyzed by this method. The proteins present in the samples were identified from the amino acid molar percentages by correlation to published amino acid composition data.
2. SCOPE OF RESEARCH
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a: Appendix , References , Author Information