JAIC 1992, Volume 31, Number 3, Article 1 (pp. 267 to 274)
JAIC online
Journal of the American Institute for Conservation
JAIC 1992, Volume 31, Number 3, Article 1 (pp. 267 to 274)

ULTRAVIOLET-FLUORESCENCE MICROSCOPY OF PAINT CROSS SECTIONS:

JOHN M. MESSINGER


ABSTRACT—Cycloheptaamylose-dansyl chloride complex, a new fluorochrome specific for proteins, has been investigated as a stain to identify artists' paint media. Its selectivity and limitations are compared to those of the following commonly available fluorescent and nonfluorescent stains: lissamine rhodamine B sulfonyl chloride, 5-fluorescein isothiocyanate, amido black, Sudan black, rhodamine B, and 2′,7′-dichlorofluorescein. The methods used to examine the characteristics of these stains were reflected visible light microscopy and ultraviolet-fluorescence microscopy. The known samples of artists' media analyzed with the stains were linseed oil, casein, tempera, glair, gelatin, rabbit skin glue, and acrylic emulsions.

Article Sections:

1. INTRODUCTION
2. EXPERIMENTAL
3. RESULTS
4. CONCLUSIONS
a: References , Author Information
Entire Article

Copyright 1992 American Institute for Conservation of Historic and Artistic Works